Hydrocarbon Solvent

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Hexane Fraction HPLC" typically refers to a high-purity, laboratory-grade solvent used as a mobile phase in High-Performance Liquid Chromatography (HPLC). Unlike pure \(n\)-hexane, which is a single straight-chain isomer, "Hexane Fraction" is often a refined mixture of six-carbon (\(C_{6}\)) hydrocarbons, including \(n\)-hexane and its isomers like isohexane (2-methylpentane). 1. Key Specifications (HPLC Grade) To meet HPLC standards, the solvent undergoes advanced purification and submicron filtration to ensure consistent analytical results. Purity Level: Typically $\geq$95% to 99.99%.Boiling Point: Approximately 68–70°C.UV Transparency: Refined for low UV absorbance to prevent baseline interference during detection.Moisture Content: Minimal water content (often $\leq$0.01%) to maintain performance in non-polar systems.Residue on Evaporation: Extremely low (e.g., \(<0.0001\%\)) to protect sensitive HPLC columns and detectors. 2. Primary Applications Normal-Phase HPLC: Essential as a non-polar mobile phase for separating hydrophobic or lipid-soluble compounds.Sample Extraction: Used to prepare specimens for pesticide analysis, food and beverage testing, and pharmaceutical research.Pharmaceutical & Environmental Labs: Used for the purification of Active Pharmaceutical Ingredients (APIs) and detection of environmental contaminants. 3. Industrial vs. HPLC Grade Feature Industrial/Technical GradeHPLC GradeCompositionMixture of \(C_{6}\) isomers and some \(C_{7}\)Controlled high-purity \(C_{6}\) fractionPurityOften $\sim\(50–85\)n$-hexane$\geq$95% up to 99.99%Primary UseOil extraction, adhesives, degreasingPrecise laboratory chromatography

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